Products & Services
Automated high throughput mutation detection
IDna Genetics Ltd is pleased to offer a new service to its customers
Automated SSCP based mutation detection
- For SNP discovery
- SNP screening
The service is built around the acquisition of an ABI 3730. instrument configured to carry out SSCP detection.
The instrument was acquired through the "Norwich Research Park Health and Life Sciences Capacity Building Initiative" more information here.
The new instrument will provide commercial, academic and healthcare scientists access to the latest technology for mutation analysis, a key area in the life sciences. This shared facility will be operated jointly by experienced staff of the John Innes Centre Genome Laboratory and IDna Genetics and provide a focal point for increased interactions between scientists from different sectors
Click to view Technical details | Service details (click again to hide details).
Technical details
SSCP detects differences in DNA sequence by examining the shape of single stranded DNA molecules as they electrophoretically migrate though a flowable polymer.
Two DNA molecules of the same size, but different sequences, will migrate through the polymer at different speeds, even if they differ by as little as a single nucleotide.
Three samples analysed by SSCP. The two DNA strands are labeled green and blue (the orange peak is a calibration marker). The upper two panels show two identical samples, the lower panel shows a clear migration difference when compared to the two upper samples, indicting the presence of a mutation.
SSCP offers speed, cost reduction, and efficiency gains in SNP discovery and mutation screening when compared to existing technologies.
SSCP
- is unaffected by nearby mutations that make SNP probe or primer design difficult with other technologies
- only needs the sequence of a gene in order to scan for mutations in introns or exons
- is the only mutation screening and SNP discovery method that is unaffected by ploidy – a major consideration in analysis of plant genomes like wheat, oilseed rape, leeks etc.
- only requires PCR amplification and separation by capillary electrophoresis, no secondary purifications or extension reactions are necessary.
Service details
We offer the following services on the instrument to match your requirements:
- Complete service – we design primers to your chosen set of genes or sequences, amplify test samples supplied by you (as tissue, seeds or DNA), separate the products by SSCP, report the polymorphic loci and the analysis of the samples
- Amplification, Separation and analysis service – you supply primers or primer designs and samples, we amplify, separate and analyse the data, report the polymorphic loci and the analysis of the samples
- Preparation, Separation and analysis – you supply labeled PCR products suitable for ABI optics , we add calibration standards, we separate the amplicons and analyse the data, report the polymorphic loci and the analysis of the samples
- Separation only – you supply labeled PCR products suitable for ABI optics with calibration standards in a heat sealed 96 or 384 place plate , we separate the amplicons and supply you the raw data files compatible with GeneMapper for you to analyse on your own computer.
Contact us for pricing tailored to your needs and size of programme.
